Research -

Toxicant induced stress and death in C2C12 cells

To investigate chemically induced cell stress, cell death and differentiation responses in C2C12 cells using reporter cell lines, cell painting and transcriptomics.

Making reporter cell lines that allow detection of caspase activity and activation of different cell stress pathways in live cells as they are induced to either die or differentiate.
Describing toxicant induced changes in the reporter cell lines over time an at different concentrations.
Transcriptomics of dying and differentiating cells and analysis of relationships between caspase activity, stress pathway activation and gene expression.

To investigate caspase-dependent and caspase-independent DNA damage in C2C12 cell death and differentiation.

Detecting caspase activity in live cells as they are induced to either die or differentiate.
Transcriptomics and proteomics of dying and differentiating cells and analysis of relationships between caspase activity and gene expression.

To identify molecular signatures associated with either cell survival, differentiation or cell death.

Mining toxicological substructural alerts.
Evaluating different modelling approaches using legacy data.
The quantitative data from the cell stress response reporters and cell death assays will be used to establish computational biology models for stress response activation of individual stress pathways for different cell types and activation of cell death processes.
The new data sets generated from the other WPs metadata will be analysed with algorithms previously designed to identify toxicant induced signalling pathways.
The utility of the built systems in determining the most probable chemical induced cell fates will be assessed by means of prospective in vitro cell fate experiments.